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Insomnia and sleep quality were assessed using the Athens Insomnia Scale and Pittsburgh Sleep Quality Index, respectively

Maintenance dosing (1-2mg 2-3x weekly or daily topical) sustains improvements indefinitely, positioning GHK-Cu as a long-term anti-aging strategy rather than a one-time fix

GHK-Cu is found naturally in human plasma, saliva, and urine, and its concentration in plasma declines significantly with age, from approximately 200 ng/ml at age 20 to around 80 ng/ml by age 60

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