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While a few human trials have occurred, most studies involve laboratory animals

ACTH, TSH, and glucagon may not activate adenylyl cyclase in vivo since the concentration of each hormone required in vitro is much higher than is found in the circulation

The organic layer was dried over anhydrous Na 2 SO 4 , filtered, concentrated in vacuo, and purified by silica gel column chromatography to give light-yellow oil pruducts 2,2-(propane-2,2-diylbis(sulfanediyl))bis(ethan-1-ol)

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