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Data processing, including sample demultiplexing, alignment, filtering, and UMI counting, was performed using the Cell Ranger Single-Cell Software Suite (v5.0.1

Senescent cells, which have permanently ceased to divide in response to stress, secrete inflammatory molecules that increase tissue damage, impair healing, and accelerate disease severity

Reconstituted solution storage requires 2-8C refrigeration with critical light protection (keep in dark or wrapped in foil), in original vial with tight seal, avoiding repeated temperature cycling and never freezing reconstituted solution

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Funding This work was financially supported by the National Key R&D Program of China to D.Z