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To amplify the whole core region of HCV genotype 1 (573 bp, H77 positions 342914), three different strategies were used

9 NCBI Bookshelf Site RotationInjection-site rotation to reduce local irritation and lipohypertrophy

Off-period of 2 to 3 months between cycles in the published structure

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If your available syringes can't measure the required volume, adjust target concentration to fit syringe capabilities rather than compromising water volume accuracy