Marcus Conrad, Helmholtz Munich) were maintained in DMEM (Life Technologies) supplemented with 10% (v/v) bovine calf serum (Life Technologies) and 50 U ml 1 of penicillin and streptomycin (Life Technologies)
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Stably infected cell lines were constructed by lentiviral vectors, and overexpression of PRKN inhibited the protein expression of SREBF2 without affecting its mRNA expression, and inhibited both mRNA and protein expression of ACAT2 (Supplementary Fig
AsGGT2-5-RACE-RT (5-[Phos]TGAGCTCGTAA ACTC-3), AsGGT2-5-RACE-F1 (5-TGTGCGACGGTATCCG ATCA-3), AsGGT2-5-RACE-F2 (5-CTCAATCCAATTCAACCT AGAC-3), AsGGT2-5-RACE-R1 (5-CATTGTGCAGCGGACGA TAG-3), and AsGGT2-5-RACE-R2 (5-GGTTCCATTAATGTGG AGGAG-3) for AsGGT2
Rosuvastatin adjunctive therapy for rifampicin-susceptible pulmonary tuberculosis: a phase 2b, randomised, open-label, multicentre trial