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from tissue
keeping cysteine residues in a reduced state
and has a cytotoxic effect on the central nervous system
Because IPTG is not metabolized by the cell
followed by heat inactivation or organic extraction to remove the protease itself
Nickel Agarose Beads (High Density) Size:500 mL from tissueGoldBios Nickel Agarose Beads (High Density) are a ready to use immobilized metal affinity chromatography resin for rapid purification of His tagged recombinant proteins. The resin provides a binding capacity greater than 25 mg mL when evaluated with an approximately 60 kDa protein, making it well suited for experiments that require increased protein yield from a limited resin volume. Proteins can be purified under native conditions when preservation
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