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Immunofluorescence and immunohistochemistry For immunofluorescence (IF) studies, fixed testicular tissue was embedded in optimal cutting temperature (OCT) solution

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In addition, a higher proportion of youths who first smoked a whole cigarette when they were 12 years old or younger reported becoming regular smokers at some time (53.3%), compared with the proportion of youths who first smoked a whole cigarette when they were 13 years old or older (31.2%)