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After filtering, 5 L of supernatant was injected onto a Microsorb-MV column (C18, 150 mm 5.6 mm, 5 micron) using an Ultimate 3000 UHPLC system (Thermo Fisher)

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Shorter virions produced by the encapsidation of subgenome-sized RNA are usually a minor component of the virion population, although virions of two species produce an abundant short virion of 3234 nm