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Similar content being viewed by others Introduction Extensive research, spanning several decades, on tobacco smoking has shown that cigarette usage during pregnancy can lead to detrimental effects, some of which could manifest starting before birth (such as fetal death and growth restriction), whereas other issues become evident as early as birth [1, 2]
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This can be negative in some circumstances
Quantitative Real Time-PCR analysis Due to the difficulty of growing large numbers of mouse AEC cells, quantitative real-time RT-PCR, which only requires small amounts of RNA, was chosen to determine mouse iNOS mRNA levels and -actin (internal standard)
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