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Samples were stained for AIF and EndoG with the same primary antibodies as for western blotting, followed by Alexa Fluor 488 (A-21206, ThermoFisher Scientific, Carlsbad, CA) labeled anti-rabbit antibodies
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[3] In the United States alone, about 480,000 individuals die annually due to smoking [7] [8] and a 2004 study estimated that as much as one-third of China's male population will have significantly shortened lifespans due to smoking
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