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Western blotting After 1 h of WTS exposure, HBECs were washed with ice cold PBS and lysed with buffer containing 25 mM Tris, 150 mM NaCl, 1% NP-40, 1% Sodium deoxycholate pH 7.4, protease inhibitors (cOmplete, EDTA-free

The scientists included a range of flavors and analyzed each for the presence/amount of endotoxins (toxins inside a bacterial cell that are released when the cell disintegrates) and glucan, a component of fungal cell walls