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The move was part of FDAs new Youth Tobacco Prevention Plan
This design was selected because the estimate of within-arm standard deviation (SD) from the external pilot study had a wide confidence interval and the interim analysis would allow for a more accurate estimation of the SD as a basis for assessing whether the expected effect size could be feasibly demonstrated
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After fixation, the cells were washed again with PBS and incubated overnight at 4 C with primary antibodies in incubation buffer (0.1% saponin, 0.1% Tween-20, and 5% FBS in PBS)
Link to survey: Reading the results and conclusions of this study leaves no doubt that the media has twisted the truth in its articles
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