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Correlative light and electron microscopy Choose seedlings displaying strong fluorescence, then subject them to high-pressure freezing followed by freeze substitution at 80 C for 24 h in anhydrous acetone containing 0.25% glutaraldehyde and 0.1% uranyl acetate

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As part of the MECHANISMS study, a sociodemographic questionnaire was completed by all students who received the smoking prevention interventions [43] (see Electronic Supplementary Material 2 which details their sociodemographic characteristics)

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