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Why Confirmation Testing Matters If an initial screen is positive, certified laboratories perform confirmatory testing using: Gas Chromatography-Mass Spectrometry (GC-MS) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) These confirmatory tests are highly specific and eliminate false positives caused by cross-reactivity

21 : traditional PCR followed by enzymatic restriction using StyI-HF (New England BioLabs, MA, USA)