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Gillian L.Schauer, Zachary R.Rosenberry, Erica N.Peters
At day 18 in vitro (DIV18), we stimulated the neurons using either field stimulation (3-s trains consisting of 32 1-ms 50-mA pulses at 20 Hz for a total of 1 or 5 min), or via replacement of culture medium with medium of identical composition (this mechanically stimulates the cultures and also provides a fresh source of glutamate)
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doi: 10.3390/ijerph6020445 22 WengMWLeeHWParkSHHuYWangHTChenLCet al
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