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These data are further confirmed by fluorescent 3D imaging of mitochondrial distribution in BEAS-2B cells upon long-term 10% CSE exposure, showing increased numbers of branched mitochondria (Online Additional file 1)

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Our findings should provide reassurance to smokers that it is okay to use whatever nicotine doses they find helpful. First study to explore new way to dose nicotine When nicotine replacement treatment was first evaluated in the 1970s, low doses were used because of concerns about toxicity and addictiveness