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341F_ADA_5B 5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG CTACT CCTACGGGAGGCAGCAG 3 534R_ADA_5B 5 GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG CATCT ATTACCGCGGCTGCTGGC 3 The optimized PCR conditions included the following: initial denaturation at 94C for 5 min, followed by 35 cycles of denaturation at 94C for 30 s, annealing at 69C for 30 s, extension at 72C for 30 s, and a final extension cycle at 72C for 5 min

doi: 10.1037/a0038178 87 HirschiT

Where they grow them in colder regions, they more commonly germinate them in cold frames

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A few years back, when temperatures climbed into the 80s, he retreated the start of the race into March