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He moved from Los Angeles back home to Alexandria, Virginia, bought a house in a tree-lined suburban subdivision not far from historical Old Town and installed a basement studio

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The thermal cycling steps were: 50 C for 2 min, 95 C for 10 min, and 40 cycles of 95 C for 15 s and 60 C for 1 min, and qPCR was performed on a Step-One-Plus real-time PCR machine (Applied Biosystems) using the StepOne Software v2.3

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Some outdoor areas may be more relaxed, but you should still respect people around you